Interleukin-6 expression after infectious bronchitis virus infection in chickens

Viral Immunol. 2007 Sep;20(3):479-86. doi: 10.1089/vim.2006.0109.

Abstract

Viral infections in chickens pose a major health threat to the poultry industry. Infectious bronchitis virus (IBV) usually causes respiratory disease; however, the disease severity is influenced by the genotype of the chicken and the IBV strain involved. Nephropathogenic strains of IBV, such as the Australian T strain, can cause high mortalities due to kidney failure characterized by mononuclear cell infiltration and inflammation. In a previous study, a line of specific pathogen-free chickens, the S-line, was shown to be susceptible to high mortalities from IBV infection. The cause of these high mortalities is unknown but it is suspected that differential cytokine expression may play a role. With this in mind, we decided to study the role of the proinflammatory cytokine interleukin (IL)-6 during infection to determine its contribution to nephritis and influence on disease susceptibility. To investigate this, we infected the susceptible S-line and the more disease-resilient HWL line with the T strain of IBV and measured their cytokine response levels. In both lines of birds, IL-6 mRNA levels were elevated in the kidneys at 4 d postinfection. However, in S-line chickens, these levels were 20 times higher than those in the HWL chickens. In addition, S-line birds also showed three times higher serum IL-6 levels than HWL birds after IBV infection. These findings suggest that IL-6 may play a role in IBV-induced nephritis and may open an avenue to develop alternative strategies, such as the use of antiinflammatory cytokines, to overcome the nephropathogenic effects of IBV.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens*
  • Coronavirus Infections / complications
  • Coronavirus Infections / immunology
  • Coronavirus Infections / veterinary*
  • Disease Susceptibility / immunology
  • Gene Expression
  • Infectious bronchitis virus / immunology*
  • Interleukin-6 / biosynthesis*
  • Interleukin-6 / blood
  • Interleukin-6 / genetics
  • Kidney / immunology
  • Kidney / pathology
  • Nephritis / immunology
  • Poultry Diseases / immunology*
  • RNA, Messenger / biosynthesis
  • Specific Pathogen-Free Organisms
  • Transcription, Genetic

Substances

  • Interleukin-6
  • RNA, Messenger