Abstract
Bradyrhizobium strains, isolated in New Caledonia from nodules of the endemic legume Serianthes calycina growing in nickel-rich soils, were able to grow in the presence of 15 mM NiCl2. The genomes of these strains harbored two Ni resistance determinants, the cnr and nre operons. By constructing a cnrA mutant, we demonstrated that the cnr operon determines the high nickel resistance in Bradyrhizobium strains.
MeSH terms
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Anti-Bacterial Agents / metabolism
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Anti-Bacterial Agents / toxicity*
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Bacterial Proteins / genetics
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Bradyrhizobium / drug effects*
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Bradyrhizobium / genetics*
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Bradyrhizobium / isolation & purification
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Drug Resistance, Bacterial / genetics*
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Fabaceae / microbiology
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Molecular Sequence Data
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Mutagenesis
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Mutant Proteins / genetics
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New Caledonia
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Nickel / metabolism
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Nickel / toxicity*
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Operon
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Sequence Analysis, DNA
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA, Bacterial
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Mutant Proteins
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nickel chloride
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Nickel
Associated data
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GENBANK/AM179845
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GENBANK/AM179846
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GENBANK/AM179847
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GENBANK/AM179848
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GENBANK/AM179851
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GENBANK/AM236914
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GENBANK/AM236915
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GENBANK/AM236916
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GENBANK/AM260684
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GENBANK/AM260685
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GENBANK/AM260686
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GENBANK/AM260687
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GENBANK/AM260688