Constitution and quantity of lysis buffer alters outcome of reverse phase protein microarrays

Proteomics. 2007 Nov;7(22):4066-8. doi: 10.1002/pmic.200700484.

Abstract

Application of novel technology to clinical samples requires optimization of procedures. Reverse phase protein lysate arrays use femtomolar quantities of tissue lysate from clinical samples with which to profile biochemical events happening in the tumor. We analyzed the effects of different tissue solubilization buffers on frozen ovarian tumor samples in order to identify the system with the best signal intensity dynamic range, reproducibility, tissue solubility, and signal consistency. A modified RIPA-like buffer supplemented with DTT and SDS was deemed optimal.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Buffers
  • Dithiothreitol / chemistry*
  • Female
  • Humans
  • Neoplasm Proteins / analysis*
  • Ovarian Neoplasms / diagnosis*
  • Protein Array Analysis / methods*
  • Proteomics / methods*
  • Reproducibility of Results
  • Sodium Dodecyl Sulfate / chemistry*
  • Solubility

Substances

  • Buffers
  • Neoplasm Proteins
  • Sodium Dodecyl Sulfate
  • Dithiothreitol