Appropriate expression of imprinted genes on mouse chromosome 12 extends development of bi-maternal embryos to term

FEBS Lett. 2007 Nov 13;581(27):5178-84. doi: 10.1016/j.febslet.2007.10.004. Epub 2007 Oct 12.

Abstract

Recently, we reported that the restored regulation of imprinted gene expression from two regions -H19 differentially methylated region (H19-DMR) and intergenic germline-derived DMR (IG-DMR) - is sufficient for accomplishing full-term development in mice. In the present study, we determined the developmental ability of the bi-maternal embryos (BMEs) containing the non-growing oocyte genome with the IG-DMR deletion (ng(Deltach12)) and fully-grown (fg) oocyte genome. Foetuses derived from ng(Deltach12)/fg BMEs were alive at E19.5 but could not survive further. Comparison with BMEs derived from Igf2+/- ng/fg genomes suggests that bi-allelic H19 expression might be involved in foetal development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Methylation
  • Embryonic Development / genetics*
  • Female
  • Gene Deletion
  • Gene Expression Regulation, Developmental*
  • Genomic Imprinting*
  • Lung / abnormalities
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Nuclear Transfer Techniques
  • Oocytes / metabolism
  • Phenotype
  • Pregnancy
  • RNA, Long Noncoding
  • RNA, Untranslated / genetics

Substances

  • H19 long non-coding RNA
  • RNA, Long Noncoding
  • RNA, Untranslated