Abstract
We previously showed that a trans-splicing ribozyme reprograms tumor-related genes at the mRNA level, resulting in the expression of therapeutic genes and that this approach can be efficiently employed to target specific molecules. Here, we show that trans-splicing ribozyme technology can be applied in molecular imaging of specific RNA expression in living animals. We exemplify this concept successfully by imaging mouse cytoskeleton-associated protein 2 (mCKAP2) expression in intrahepatic tumor nodules using systemically delivered adenovirus harboring mCKAP2-specific trans-splicing ribozyme.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenoviridae / genetics*
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Animals
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Cell Line, Tumor
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Cytoskeletal Proteins / genetics
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Cytoskeletal Proteins / metabolism
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Diagnostic Imaging
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Disease Models, Animal
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Neoplastic*
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Genes, Reporter
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Humans
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Male
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Mice
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Mice, Inbred C57BL
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Neoplasm Transplantation
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Neoplasms / diagnosis
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Neoplasms / genetics*
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RNA, Catalytic / genetics
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RNA, Catalytic / metabolism*
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RNA, Messenger / analysis*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Trans-Splicing / genetics*
Substances
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CKAP2 protein, mouse
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Cytoskeletal Proteins
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RNA, Catalytic
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RNA, Messenger