Abstract
The monoclonal antibody TU-20 and its scFv fragment were radiolabeled with 125 I in order to develop new imaging agents against the specific neuronal marker III beta-tubulin. The reaction via chloramine-T using thiosulfate as a stopping reductant was determined as the most convenient way for radioiodination. The preserved immunological properties of radioiodinated species were estimated by ELISA, electrophoresis, and immunohistochemistry with autoradiography. Biodistribution studies revealed a different behavior of radioiodinated TU-20 and its scFv.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / chemistry*
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Antibodies, Monoclonal / immunology
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Antibodies, Monoclonal / pharmacokinetics
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Autoradiography
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Immunoconjugates / chemistry
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Immunoconjugates / immunology
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Immunoconjugates / pharmacokinetics
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Immunoglobulin Fragments / chemistry
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Immunoglobulin Fragments / immunology
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Immunohistochemistry
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Iodine Radioisotopes / chemistry*
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Isotope Labeling
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Male
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Mice
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Mice, Inbred C57BL
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Peripheral Nervous System Diseases / diagnostic imaging*
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Peripheral Nervous System Diseases / metabolism
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Radionuclide Imaging
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Tissue Distribution
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Tubulin / immunology*
Substances
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Antibodies, Monoclonal
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Immunoconjugates
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Immunoglobulin Fragments
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Iodine Radioisotopes
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Tubulin
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beta3 tubulin, mouse