Abstract
D-Cycloserine (DCS) is only used with multidrug-resistant strains of tuberculosis because of serious side effects. DCS is known to inhibit cell wall biosynthesis, but the in vivo lethal target is still unknown. We have applied NMR-based metabolomics combined with principal component analysis to monitor the in vivo effect of DCS on Mycobacterium smegmatis. Our analysis suggests DCS functions by inhibiting multiple protein targets.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Alanine / physiology
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Alanine Racemase / biosynthesis
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Alanine Racemase / genetics
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Alanine Racemase / metabolism
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Alanine Racemase / physiology*
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Antibiotics, Antitubercular / pharmacology*
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Cycloserine / pharmacology*
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Drug Resistance, Multiple, Bacterial
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Magnetic Resonance Spectroscopy*
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Mycobacterium smegmatis / drug effects
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Mycobacterium smegmatis / enzymology*
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Mycobacterium smegmatis / genetics
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Mycobacterium smegmatis / growth & development
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Peptidoglycan / biosynthesis
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Proteome / metabolism*
Substances
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Antibiotics, Antitubercular
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Peptidoglycan
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Proteome
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Cycloserine
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Alanine Racemase
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Alanine