Abstract
Ha-AP10 is a basic antifungal peptide from sunflower seeds (Helianthus annuus antifungal peptide of 10 kDa) belonging to the family of plant lipid transfer proteins. We report here its expression in E. coli [Glutathione S-transferase (GST) system] and its phosphorylation by endogenous membrane-bound calcium-dependent protein kinases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antifungal Agents / metabolism
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Antigens, Plant / biosynthesis
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Carrier Proteins / biosynthesis*
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Cloning, Molecular
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Escherichia coli / metabolism*
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Molecular Sequence Data
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Plant Proteins / biosynthesis*
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Protein Kinases / metabolism*
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Recombinant Fusion Proteins / biosynthesis
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Sequence Alignment
Substances
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Antifungal Agents
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Antigens, Plant
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Carrier Proteins
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Ha-AP10 protein, Helianthus annuus
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Plant Proteins
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Recombinant Fusion Proteins
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lipid transfer proteins, plant
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Protein Kinases
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calcium-dependent protein kinase