Abstract
The aim of this study was to develop a highly sensitive human papillomavirus type 31 (HPV31) neutralization assay based on the production of pseudovirions carrying luciferase. Neutralizing antibodies against HPV31 were investigated in a set of HPV31 monoclonal antibodies and in women with evidence of HPV31 infection. Neutralizing antibodies were detected in 78% of subjects with a positive enzyme-linked immunosorbent assay.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibodies, Monoclonal / immunology
-
Antibodies, Viral / analysis*
-
Antibodies, Viral / immunology
-
COS Cells
-
Cell Line
-
Chlorocebus aethiops
-
Enzyme-Linked Immunosorbent Assay / methods
-
Female
-
Humans
-
Insecta
-
Luciferases / biosynthesis
-
Luciferases / chemistry*
-
Luciferases / genetics
-
Neutralization Tests
-
Papillomaviridae / immunology*
-
Papillomaviridae / isolation & purification
-
Papillomavirus Infections / diagnosis*
-
Papillomavirus Infections / immunology
-
Papillomavirus Infections / virology
-
Virion / enzymology*
-
Virion / genetics
-
Virus Assembly / physiology
Substances
-
Antibodies, Monoclonal
-
Antibodies, Viral
-
Luciferases