Activity-based fluorescent reporters for monoamine oxidases in living cells

Chem Commun (Camb). 2007 Nov 28:(44):4647-9. doi: 10.1039/b713190a. Epub 2007 Oct 9.

Abstract

Monoamine Oxidase Reporters 1 and 2 (MR1 and MR2) are substrate-tethered activity probes that utilize a tandem amine oxidation/beta-elimination mechanism to detect MAO chemistry in vitro and in living cells directly and specifically without the need for additional enzymes or other activating reagents.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Enzyme Activation
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Molecular Structure
  • Monoamine Oxidase / chemistry*
  • PC12 Cells
  • Rats
  • Time Factors

Substances

  • Fluorescent Dyes
  • Monoamine Oxidase