Abstract
In recent years, molecular genetic approaches to the study of the disease pathogenesis of Staphylococcus aureus have resulted in many new biological insights. I describe methods used for targeted disruption of staphylococcal genes leading to loss of gene function, important for studies of staphylococcal proteins and their role in virulence.
MeSH terms
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Alleles
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Cloning, Molecular
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Drug Resistance, Bacterial / genetics
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Gene Deletion
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Gene Targeting
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Genes, Bacterial
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Mutagenesis
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Plasmids / genetics
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Polymerase Chain Reaction / methods
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Staphylococcus Phages / genetics
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Staphylococcus aureus / drug effects
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Staphylococcus aureus / genetics*
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Staphylococcus aureus / pathogenicity*
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Temperature
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Transformation, Genetic
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Virulence / genetics