Molecular characterization of hepatitis A virus in children with fulminant hepatic failure in Argentina

Liver Int. 2008 Jan;28(1):47-53. doi: 10.1111/j.1478-3231.2007.01634.x. Epub 2007 Nov 19.

Abstract

Background: Hepatitis A infection, a vaccine-preventable disease, is an important cause of fulminant hepatic failure (FHF) in children in Argentina. Universal vaccination in 1-year-old children was implemented in June 2005. The limited studies about the correlation between the characteristics of the hepatitis A virus (HAV) and FHF have been carried out in adults.

Methods: Samples from 41 children with FHF were studied from September 2003 to January 2006 and HAV RNA was detected, sequenced and analysed in the 5' non-coding region and VP1/2A region.

Results: Eighteen HAV strains were characterized and found to be different at the nucleotide level from the self-limited acute infection strains that have been circulating in Argentina with no temporal or geographical pattern. They did not form a genetic cluster, but some of them were identical in the largest fragment characterized and some of them seemed to be more closely related in time and/or geographically.

Conclusion: Our results suggest that viral factors could be involved in the severity of the clinical presentation of HAV infection in children in Argentina.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Argentina
  • Base Sequence
  • Child
  • Child, Preschool
  • Feces / virology
  • Hepatitis A / complications*
  • Hepatitis A / genetics
  • Hepatitis A Virus, Human / genetics*
  • Humans
  • Infant
  • Liver Failure, Acute / etiology*
  • Liver Failure, Acute / virology
  • Molecular Sequence Data
  • Phylogeny*
  • RNA / genetics
  • Sequence Analysis, RNA

Substances

  • RNA