Abstract
We evaluated a two-step algorithm for detection of Clostridium difficile in 1,468 stool specimens. First, specimens were screened by an immunoassay for C. difficile glutamate dehydrogenase antigen (C.DIFF CHEK-60). Second, screen-positive specimens underwent toxin testing by a rapid toxin A/B assay (TOX A/B QUIK CHEK); toxin-negative specimens were subjected to stool culture. This algorithm allowed final results for 92% of specimens with a turnaround time of 4 h.
MeSH terms
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Adult
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Algorithms
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Antigens, Bacterial / analysis
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Bacterial Toxins / analysis
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Bacteriological Techniques / methods*
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Clostridioides difficile / isolation & purification*
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Enterocolitis, Pseudomembranous / diagnosis*
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Enterotoxins / analysis
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Feces / chemistry
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Feces / microbiology
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Glutamate Dehydrogenase / analysis
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Humans
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Immunoassay / methods*
Substances
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Antigens, Bacterial
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Bacterial Toxins
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Clostridium difficile lethal toxin B
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Enterotoxins
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tcdA protein, Clostridium difficile
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Glutamate Dehydrogenase