Abstract
The sigG gene of Mycobacterium tuberculosis was disrupted by homologous recombination, and the genes regulated by SigG were examined by real-time reverse-transcription PCR and microarray studies. The SigG consensus promoter recognition sequence was identified as GCGNGT-N15-18-CGANCA. A DeltasigG mutant was found to be more resistant to mitomycin C treatment than the wild-type strain, indicating that it may be involved in the SOS response in M. tuberculosis.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Base Sequence
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Cell Line
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DNA-Directed RNA Polymerases / genetics
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DNA-Directed RNA Polymerases / metabolism*
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Gene Expression Regulation, Bacterial*
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Heat-Shock Response*
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Humans
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Macrophages, Alveolar / microbiology
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Mice
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Molecular Sequence Data
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Mycobacterium tuberculosis / metabolism
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Mycobacterium tuberculosis / pathogenicity
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Mycobacterium tuberculosis / physiology*
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Oligonucleotide Array Sequence Analysis
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Promoter Regions, Genetic
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Recombination, Genetic
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Reverse Transcriptase Polymerase Chain Reaction
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SOS Response, Genetics*
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Sigma Factor / genetics
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Sigma Factor / metabolism*
Substances
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Bacterial Proteins
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Sigma Factor
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RNA polymerase sigma 70
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DNA-Directed RNA Polymerases