The analysis of thiocarbamides by gas chromatography/negative-ion chemical-ionization mass spectrometry

Rapid Commun Mass Spectrom. 1991 Mar;5(3):141-2. doi: 10.1002/rcm.1290050311.

Abstract

Thiocarbamides were converted to their di-N-pentafluorobenzyl (PFB) derivatives and analysed by gas chromatography/negative-ion chemical-ionization mass spectrometry with methane as reagent gas. The PFB derivatives of the 2-thiouracils gave mass spectra in which the ion current was carried largely by an ion arising from [M-PFB]-. The derivative was used in the determination of the uptake and metabolism of thiocarbamides by cultures of melanoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gas Chromatography-Mass Spectrometry / methods
  • Melanoma / metabolism*
  • Methimazole / pharmacokinetics
  • Methylthiouracil / pharmacokinetics*
  • Propylthiouracil / pharmacokinetics*
  • Thiouracil / analogs & derivatives*
  • Thiouracil / pharmacokinetics*
  • Tumor Cells, Cultured

Substances

  • Methimazole
  • Thiouracil
  • Propylthiouracil
  • benzylthiouracil
  • Methylthiouracil