Complete 1H and 13C NMR assignments of three new polyhydroxylated sterols from the South China Sea gorgonian Subergorgia suberosa

Magn Reson Chem. 2007 Dec;45(12):1088-91. doi: 10.1002/mrc.2102.

Abstract

Three new polyhydroxylated sterols, 3beta,6alpha,11,20beta,24-pentahydroxy- 9,11-seco-5alpha-24-ethylcholest-7,28-diene-9-one (1), 3-(1',2'-ethandiol)-24- methylcholest-8(9),22E-diene-3beta,5alpha,6alpha,7alpha,11alpha-pentaol (2), 24-methylcholest-7,22 E-diene-3beta,5alpha,6beta,25-tetraol (3) together with five known sterols, were isolated from the EtOH/CH2Cl2 extract of the South China Sea gorgonian Subergorgia suberosa. The complete assignments of the 1H and 13C NMR chemical shifts for these new compounds were achieved by means of 1D and 2D NMR techniques, including HSQC, HMBC, 1H--1H COSY, and NOESY spectra.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anthozoa / chemistry*
  • Carbon Isotopes
  • China
  • Magnetic Resonance Spectroscopy / methods*
  • Magnetic Resonance Spectroscopy / standards*
  • Molecular Conformation
  • Protons
  • Reference Standards
  • Sensitivity and Specificity
  • Sitosterols / chemistry*
  • Sitosterols / isolation & purification
  • Species Specificity
  • Stereoisomerism
  • Sterols / chemistry*
  • Sterols / isolation & purification

Substances

  • 24-methylcholest-7,22 E-diene-3beta,5alpha,6beta,25-tetraol
  • 3-(1',2'-ethandiol)-24-methylcholest-8(9),22E-diene-3beta,5alpha,6alpha,7alpha,11alpha-pentaol
  • 3beta,6alpha,11,20beta,24-pentahydroxy- 9,11-seco-5alpha-24-ethylcholest-7,28-diene-9-one
  • Carbon Isotopes
  • Protons
  • Sitosterols
  • Sterols