Abstract
New putative antigenic peptides corresponding to the N- and C-terminal of the E2 envelope protein of GBV-C/HGV were synthesized using solid-phase chemistry. The antigens were obtained in linear and chimeric forms with the main aim of improving the sensitivity of the enzyme immunoassays. Furthermore, CD and FTIR have been used in conjunction to characterize their conformational changes showing that the chimeric peptide presents a more ordered secondary structure than its parent peptides.
MeSH terms
-
Amino Acid Sequence
-
Circular Dichroism
-
Enzyme-Linked Immunosorbent Assay
-
Epitopes / immunology
-
Flaviviridae Infections / diagnosis*
-
Flaviviridae Infections / virology
-
GB virus C / immunology*
-
Hepatitis, Viral, Human / diagnosis*
-
Hepatitis, Viral, Human / virology
-
Humans
-
Molecular Sequence Data
-
Peptides / chemical synthesis*
-
Peptides / chemistry
-
Peptides / immunology
-
Protein Structure, Secondary
-
ROC Curve
-
Serologic Tests / methods*
-
Spectroscopy, Fourier Transform Infrared
-
Viral Envelope Proteins / chemistry
Substances
-
Epitopes
-
Peptides
-
Viral Envelope Proteins