Structure of the mature ectodomain of the human receptor-type protein-tyrosine phosphatase IA-2

J Biol Chem. 2008 Feb 22;283(8):4674-81. doi: 10.1074/jbc.M708144200. Epub 2007 Nov 29.

Abstract

IA-2 (insulinoma-associated protein 2) is a protein-tyrosine phosphatase receptor located in secretory granules of neuroendocrine cells. Initially, it attracted attention due to its involvement in the autoimmune response associated to diabetes. Later it was found that upon exocytosis, the cytoplasmic domain of IA-2 is cleaved and relocated to the nucleus, where it enhances the transcription of the insulin gene. A concerted functioning of the whole receptor is to be expected. However, very little is known about the structure and function of the transmembrane and extracellular domains of IA-2. To address this issue, we solved the x-ray structure of the mature ectodomain of IA-2 (meIA-2) to 1.30A resolution. The fold of meIA-2 is related to the SEA (sea urchin sperm protein, enterokinase, agrin)) domains of mucins, suggesting its participation in adhesive contacts to the extracellular matrix and providing clues on how this kind of molecule may associate and form homo- and heterodimers. Moreover, we discovered that meIA-2 is self-proteolyzed in vitro by reactive oxygen species, suggesting the possibility of a new shedding mechanism that might be significant in normal function or pathological processes. Knowledge of meIA-2 structure should facilitate the search of its possible ligands and molecular interactions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Autoimmunity / immunology
  • Crystallography, X-Ray
  • Diabetes Mellitus / genetics
  • Diabetes Mellitus / immunology
  • Exocytosis / genetics
  • Exocytosis / immunology
  • Extracellular Matrix
  • Humans
  • Models, Molecular*
  • Protein Folding*
  • Reactive Oxygen Species / chemistry
  • Reactive Oxygen Species / immunology
  • Receptor-Like Protein Tyrosine Phosphatases, Class 8 / chemistry*
  • Receptor-Like Protein Tyrosine Phosphatases, Class 8 / genetics
  • Receptor-Like Protein Tyrosine Phosphatases, Class 8 / immunology
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Structural Homology, Protein
  • Structure-Activity Relationship

Substances

  • Reactive Oxygen Species
  • Recombinant Proteins
  • PTPRN protein, human
  • Receptor-Like Protein Tyrosine Phosphatases, Class 8

Associated data

  • PDB/2QT7