Selective isolation of bacterial DNA from human clinical specimens

Hans-Peter Horz; Sebastian Scheer; Frank Huenger; Morgana E Vianna; Georg Conrads

doi:10.1016/j.mimet.2007.10.007

Selective isolation of bacterial DNA from human clinical specimens

J Microbiol Methods. 2008 Jan;72(1):98-102. doi: 10.1016/j.mimet.2007.10.007. Epub 2007 Nov 28.

Authors

Hans-Peter Horz¹, Sebastian Scheer, Frank Huenger, Morgana E Vianna, Georg Conrads

Affiliation

¹ Division of Oral Microbiology and Immunology, RWTH Aachen University Hospital, Germany. [email protected]

PMID: 18053601
DOI: 10.1016/j.mimet.2007.10.007

Abstract

We evaluated two DNA preparation strategies (MolYsis, Molzym GmbH & Co. KG, Bremen, Germany) and Pureprove, SIRS-Lab GmbH, Jena, Germany) to selectively extract bacterial DNA from human clinical samples. By testing 16 oral samples we found that human DNA could be largely eliminated while detectable levels of bacterial DNA were obtained with all samples. Both approaches hold great potential for microbial diagnostic systems.

Publication types

Evaluation Study

MeSH terms

Adult
Bacteria / isolation & purification
Bacteroidaceae Infections / microbiology
DNA / analysis
DNA / isolation & purification
DNA Primers
DNA, Bacterial / analysis
DNA, Bacterial / isolation & purification*
Dental Caries / microbiology*
Humans
Periodontitis / microbiology*
Polymerase Chain Reaction / methods
Porphyromonas gingivalis / genetics
Porphyromonas gingivalis / isolation & purification
Reagent Kits, Diagnostic*
Streptococcal Infections / microbiology
Streptococcus mutans / genetics
Streptococcus mutans / isolation & purification

Substances

DNA Primers
DNA, Bacterial
Reagent Kits, Diagnostic
DNA