Human mulipotential mesenchymal/stromal stem cells are derived from a discrete subpopulation of STRO-1bright/CD34 /CD45(-)/glycophorin-A-bone marrow cells

Andrew C W Zannettino; Sharon Paton; Angela Kortesidis; Fiona Khor; Silviu Itescu; Stan Gronthos

doi:10.3324/haematol.11691

Human mulipotential mesenchymal/stromal stem cells are derived from a discrete subpopulation of STRO-1bright/CD34 /CD45(-)/glycophorin-A-bone marrow cells

Haematologica. 2007 Dec;92(12):1707-8. doi: 10.3324/haematol.11691.

Authors

Andrew C W Zannettino¹, Sharon Paton, Angela Kortesidis, Fiona Khor, Silviu Itescu, Stan Gronthos

Affiliation

¹ Multiple Myeloma Research Laboratory, Division of Haematology, Institute of Medical and Veterinary Science, Hanson Institute and University of Adelaide, Australia.

PMID: 18055998
DOI: 10.3324/haematol.11691

Abstract

Magnetic and flow cytometry-based methods were used to characterize clonogenic stromal cells in human bone marrow. STRO-1(bright) stromal cells were found to lack expression of CD34, CD45 and glycophorin-A markers associated with hematopoietic progenitor cells. These studies support the view that these are two distinct stem cell compartments in adult bone marrow.

MeSH terms

Antigens, CD34
Antigens, Differentiation*
Antigens, Surface
Bone Marrow
Flow Cytometry
Glycophorins
Hematopoietic Stem Cells / cytology
Humans
Immunomagnetic Separation
Leukocyte Common Antigens
Mesenchymal Stem Cells / cytology*
Multipotent Stem Cells / cytology*
Stromal Cells / cytology

Substances

Antigens, CD34
Antigens, Differentiation
Antigens, Surface
Glycophorins
STRO-1 antigen, human
Leukocyte Common Antigens
PTPRC protein, human