KCl cotransport in HbAA and HbSS red cells: activation by intracellular acidity and disappearance during maturation

J C Ellory; A C Hall; S A Ody; C E Poli de Figueiredos; S Chalder; J Stuart

doi:10.1007/978-1-4684-5985-2_5

KCl cotransport in HbAA and HbSS red cells: activation by intracellular acidity and disappearance during maturation

Adv Exp Med Biol. 1991:307:47-57. doi: 10.1007/978-1-4684-5985-2_5.

Authors

J C Ellory¹, A C Hall, S A Ody, C E Poli de Figueiredos, S Chalder, J Stuart

Affiliation

¹ Department of Physiology, University of Oxford.

PMID: 1805601
DOI: 10.1007/978-1-4684-5985-2_5

Abstract

Low intracellular pH was shown to be a potent activator of the KCl cotransport system in HbSS red cells, and in reticulocyte-rich fractions of HbAA red cells. Rheological experiments indicated that cell dehydration via the KCl cotransporter in response to low pH decreased the filterability of HbSS red cells. In vitro maturation experiments showed that the KCl cotransport system was rendered cryptic rapidly, in contrast to choline transport, and serine transport via system ASC, which disappeared much more slowly.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anemia, Sickle Cell / blood*
Biological Transport / drug effects
Bumetanide / pharmacology
Carrier Proteins / blood*
Choline / blood
Dehydration
Erythrocyte Aging*
Erythrocyte Deformability
Erythrocyte Volume
Hemochromatosis / blood
Humans
Hydrogen-Ion Concentration
Intracellular Fluid*
K Cl- Cotransporters
Osmolar Concentration
Ouabain / pharmacology
Potassium Chloride / blood*
Reticulocytes / metabolism
Serine / blood
Symporters*

Substances

Carrier Proteins
Symporters
Bumetanide
Serine
Ouabain
Potassium Chloride
Choline