Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source

Dylan M Owen; Egidijus Auksorius; Hugh B Manning; Clifford B Talbot; Pieter A A de Beule; Christopher Dunsby; Mark A A Neil; Paul M W French

doi:10.1364/ol.32.003408

Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source

Opt Lett. 2007 Dec 1;32(23):3408-10. doi: 10.1364/ol.32.003408.

Authors

Dylan M Owen¹, Egidijus Auksorius, Hugh B Manning, Clifford B Talbot, Pieter A A de Beule, Christopher Dunsby, Mark A A Neil, Paul M W French

Affiliation

¹ Chemical Biology Centre, Photonics Group, Department of Physics, Imperial College London, UK. [email protected]

PMID: 18059949
DOI: 10.1364/ol.32.003408

Abstract

We present a time-gated, optically sectioned, hyperspectral fluorescence lifetime imaging (FLIM) microscope incorporating a tunable supercontinuum excitation source extending into the UV. The system is capable of resolving the excitation spectrum, emission spectrum, and fluorescence decays in an optically sectioned image.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Collagen / chemistry
Collagen / ultrastructure
Convallaria / cytology
Image Processing, Computer-Assisted / instrumentation
Image Processing, Computer-Assisted / methods*
Microscopy, Fluorescence / instrumentation
Microscopy, Fluorescence / methods*
Microscopy, Ultraviolet / instrumentation
Microscopy, Ultraviolet / methods
Rats
Tail / cytology

Substances

Collagen