Abstract
To assess the effects of anti-TNF-alpha antibody (infliximab) in experimental steatohepatitis induced by methionine- and choline-deficient (MCD) diet. The study included thirty rats. One group received normal rat food, and two groups received MCD diet. The treatment group received a single dose intra-peritoneal infliximab (4 mg/kg), at week 8. MCD diet increased levels of AST, ALT, TNF-alpha, TGF-beta(1), tissue and plasma MDA (p < 0.05 for each). Moreover, it led to steatosis, ballooning degeneration, inflammation, fibrosis and increased actin expression, histopathologically (p < 0.05 for each). In this experimental steatohepatitis anti-TNF-alpha antibody decreased the levels of AST, ALT, TGF-beta(1) and plasma and tissue MDA (p < 0.05 for each). Moreover, inflammation, necrosis, actin expression and fibrosis decreased in anti-TNF-alpha group compared to placebo group (p < 0.05 for each). This study indicates that anti-TNF-alpha antibody is effective on necrosis, inflammation and fibrosis in the experimental model of non-alcoholic steatohepatitis, induced by MCD diet.
MeSH terms
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Actins / metabolism
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Alanine Transaminase / blood
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Animals
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Anti-Inflammatory Agents / administration & dosage
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Anti-Inflammatory Agents / pharmacology*
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Antibodies, Monoclonal / administration & dosage
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Antibodies, Monoclonal / pharmacology*
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Aspartate Aminotransferases / blood
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Choline Deficiency / complications
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Choline Deficiency / drug therapy*
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Choline Deficiency / pathology
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Diet
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Disease Models, Animal
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Fatty Liver / etiology
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Fatty Liver / pathology
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Fatty Liver / prevention & control*
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Hepatitis / etiology
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Hepatitis / pathology
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Hepatitis / prevention & control*
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Infliximab
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Injections, Intraperitoneal
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Liver / drug effects
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Liver / enzymology
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Liver / pathology
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Liver Cirrhosis / etiology
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Liver Cirrhosis / pathology
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Liver Cirrhosis / prevention & control*
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Male
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Malondialdehyde / metabolism
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Methionine / deficiency*
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Necrosis
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Rats
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Time Factors
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Transforming Growth Factor beta1 / metabolism
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Tumor Necrosis Factor-alpha / antagonists & inhibitors*
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Tumor Necrosis Factor-alpha / immunology
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Actins
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Anti-Inflammatory Agents
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Antibodies, Monoclonal
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Transforming Growth Factor beta1
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Tumor Necrosis Factor-alpha
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Malondialdehyde
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Methionine
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Infliximab
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Aspartate Aminotransferases
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Alanine Transaminase