Dual-probe system using pyrenylmethyl-modified amino-LNA for mismatch detection

Tadashi Umemoto; Patrick J Hrdlicka; B Ravindra Babu; Jesper Wengel

doi:10.1080/15257770701528255

Dual-probe system using pyrenylmethyl-modified amino-LNA for mismatch detection

Nucleosides Nucleotides Nucleic Acids. 2007;26(10-12):1261-3. doi: 10.1080/15257770701528255.

Authors

Tadashi Umemoto¹, Patrick J Hrdlicka, B Ravindra Babu, Jesper Wengel

Affiliation

¹ Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. [email protected]

PMID: 18066764
DOI: 10.1080/15257770701528255

Abstract

A dual-probe containing pyrenylmethyl amino-LNA has been developed for sensitive mismatch detection. While hybridization with complementary DNA/RNA results in very strong excimer signals, exposure to singly mismatched DNA/RNA targets results in significantly decreased excimer emission.

MeSH terms

Amines / chemistry
Base Pair Mismatch*
DNA / chemistry
DNA / genetics*
DNA Mutational Analysis / methods*
Nucleic Acid Hybridization*
Nucleic Acid Probes / chemistry*
Oligonucleotides / chemistry*
Polymorphism, Single Nucleotide
Pyrenes / chemistry
RNA / chemistry
RNA / genetics

Substances

Amines
Nucleic Acid Probes
Oligonucleotides
Pyrenes
locked nucleic acid
RNA
DNA