Are all the DNA gyrase mutations found in Mycobacterium leprae clinical strains involved in resistance to fluoroquinolones?

Stéphanie Matrat; Emmanuelle Cambau; Vincent Jarlier; Alexandra Aubry

doi:10.1128/AAC.01095-07

Are all the DNA gyrase mutations found in Mycobacterium leprae clinical strains involved in resistance to fluoroquinolones?

Antimicrob Agents Chemother. 2008 Feb;52(2):745-7. doi: 10.1128/AAC.01095-07. Epub 2007 Dec 10.

Authors

Stéphanie Matrat¹, Emmanuelle Cambau, Vincent Jarlier, Alexandra Aubry

Affiliation

¹ Université Pierre et Marie Curie-Paris 6, Paris, France.

Abstract

Mycobacterium leprae DNA gyrases carrying various mutations, previously described in clinical strains, were investigated for quinolone susceptibility by inhibition of supercoiling and DNA cleavage promotion. We demonstrated that the gyrA mutations leading to G89C or A91V confer fluoroquinolone resistance whereas the gyrB mutation leading to D205N does not.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Infective Agents / pharmacology*
DNA Gyrase / genetics*
Drug Resistance, Bacterial / genetics*
Fluoroquinolones / pharmacology*
Humans
Microbial Sensitivity Tests
Mutation*
Mycobacterium leprae / drug effects*
Mycobacterium leprae / enzymology
Mycobacterium leprae / genetics

Substances

Anti-Infective Agents
Fluoroquinolones
DNA Gyrase