Abstract
The Tob/BTG family is a group of antiproliferative proteins that contain two highly homologous regions named Box A and Box B. These proteins all associate with CCR4-associated factor 1 (Caf1), which belongs to the ribonuclease D family of deadenylases. The antiproliferative region of human Tob (residues 1-138) and intact hCaf1 were co-expressed in Escherichia coli, purified and successfully cocrystallized. The crystal belongs to the tetragonal space group I422, with unit-cell parameters a = b = 150.9, c = 113.9 A, and is estimated to contain one heterodimer per asymmetric unit. The crystal diffracted to around 2.6 A resolution.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Crystallization
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Humans
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Intracellular Signaling Peptides and Proteins / chemistry*
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / metabolism*
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Protein Binding
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Transcription Factors / chemistry*
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Tumor Suppressor Proteins / chemistry*
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Tumor Suppressor Proteins / genetics
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Tumor Suppressor Proteins / metabolism*
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X-Ray Diffraction
Substances
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CNOT8 protein, human
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Intracellular Signaling Peptides and Proteins
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TOB1 protein, human
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Transcription Factors
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Tumor Suppressor Proteins