Abstract
Hyperimmune horse anti-bothropic serum, used in serum therapy, was analyzed for its IgGT content and protective ability. IgGT was isolated through a combination of salt-mediated hydrophobic chromatography and protein A affinity chromatography. The chromatographic fractions obtained were analyzed with regard to their isotype content and protective ability. The results suggest that the protective ability of hyperimmune anti-venom serum is located mainly in the IgGT subclass.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antivenins / isolation & purification*
-
Chromatography, Affinity
-
Crotalid Venoms / immunology*
-
Crotalid Venoms / toxicity
-
Horses / immunology*
-
Immunization
-
Immunoglobulin G / isolation & purification*
-
Immunoglobulin Isotypes / isolation & purification
-
Lethal Dose 50
-
Male
-
Mice
-
Rabbits
-
Staphylococcal Protein A / immunology
Substances
-
Antivenins
-
Crotalid Venoms
-
Immunoglobulin G
-
Immunoglobulin Isotypes
-
Staphylococcal Protein A