233 cases with acute diarrhoea investigated, Clostridium difficile was isolated as a sole pathogen from 17 (7.3%) cases. The Major clinical features of these cases were watery diarrhoea (82.4%), bloody stool (17.6%), vomiting (64.8%), fever (17.6%) and abdominal pain (2.5%). Fourteen (82.4%) of 17 C difficile isolates were found to produce cytotoxin as detected by Verocell assay.