Paramagnetic relaxation-based 19f MRI probe to detect protease activity

Shin Mizukami; Rika Takikawa; Fuminori Sugihara; Yuichiro Hori; Hidehito Tochio; Markus Wälchli; Masahiro Shirakawa; Kazuya Kikuchi

doi:10.1021/ja077058z

Paramagnetic relaxation-based 19f MRI probe to detect protease activity

J Am Chem Soc. 2008 Jan 23;130(3):794-5. doi: 10.1021/ja077058z. Epub 2007 Dec 23.

Authors

Shin Mizukami¹, Rika Takikawa, Fuminori Sugihara, Yuichiro Hori, Hidehito Tochio, Markus Wälchli, Masahiro Shirakawa, Kazuya Kikuchi

Affiliation

¹ Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan.

PMID: 18154336
DOI: 10.1021/ja077058z

Abstract

A novel design principle for 19F MRI probes detecting protease activity was developed. This principle is based on 19F MRI signal quenching by the intramolecular paramagnetic effect from Gd3+. The intramolecular Gd3+ dramatically attenuated the 19F probe signal, and the paramagnetic effect was cancelled by the probe hydrolyzation by caspase-3. Using this probe, it was shown that the probe could detect caspase-3 activity spatially from a phantom image using 19F MRI.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Caspase 3 / analysis
Fluorine / chemistry*
Humans
Isotopes / chemistry
Molecular Probes / chemistry*
Nuclear Magnetic Resonance, Biomolecular*
Oligopeptides / chemical synthesis
Oligopeptides / chemistry*
Organometallic Compounds / chemical synthesis
Organometallic Compounds / chemistry*
Peptide Hydrolases / analysis*

Substances

Isotopes
Molecular Probes
Oligopeptides
Organometallic Compounds
gadolinum-DOTA-aspartyl-glutamyl-valyl-aspartic acid 4-trifluoromethoxybenzylamide
Fluorine
Peptide Hydrolases
Caspase 3