Abstract
We describe a bacterial strain developed to facilitate the determination of glycosyltransferase (GT) specificities for O antigens of known structure and gene cluster sequence. For proof of principle for the approach, the strain was used to determine the specificity of the Escherichia coli O111 O-antigen GT genes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cloning, Molecular
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Escherichia coli / enzymology*
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Escherichia coli / genetics*
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Glucosyltransferases / genetics
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Glucosyltransferases / metabolism*
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Models, Biological
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Multigene Family
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O Antigens / genetics
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O Antigens / metabolism*
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Oligonucleotides / genetics
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Species Specificity
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Substrate Specificity
Substances
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O Antigens
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Oligonucleotides
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Glucosyltransferases