Transient elastography consists of measuring the transverse local shear elastic modulus defined as local muscle hardness (LMH). It has previously been shown that LMH is correlated to muscle activity level during non-fatiguing contractions. The aim of this study was to describe how LMH and muscle activity level change during a submaximal fatiguing constant-torque protocol. Changes in gastrocnemius medialis LMH and in surface electromyographic activities (sEMG) of plantar flexors induced by a submaximal isometric plantar flexion (40% of the maximal isometric torque) until exhaustion were quantified. During the contraction, sEMG of each muscle increased (P<0.001) whereas LMH remained constant (P>0.05). Active LMH assessed during the contraction did not parallel muscle activity level changes during this type of submaximal fatigue protocol. Interestingly, LMH at rest assessed in passive conditions was higher prior to the fatiguing effort (P<0.05), rather than that assessed immediately after. Muscle and tendon viscous behaviors could imply a creep phenomenon during a prolonged isometric contraction, and our results in LMH at rest could indicate that this phenomenon induces changes in muscle intrinsic mechanical properties. Further studies are needed to examine whether it could have an influence on muscle activity levels during the contraction.