Characterization of HIV type 1 reverse transcriptase mutations in infants infected by mothers who received peripartum nevirapine prophylaxis in Jos, Nigeria

AIDS Res Hum Retroviruses. 2007 Dec;23(12):1587-92. doi: 10.1089/aid.2007.0064.

Abstract

This study was carried out to characterize HIV-1 reverse transcriptase (RT) mutations in vertically infected infants in Jos, Nigeria. DNA was extracted from peripheral blood mononuclear cells of 102 infants, aged 0 to 6 months, born to HIV-1-infected mothers who had received peripartum single-dose nevirapine prophylaxis. PCR-based diagnosis revealed that 14 infants (13.7%) were infected with HIV-1. Phylogenetic analyses of RT revealed wide viral diversity, with CRF02_AG, subtype G, subsubtype A3, CRF06_cpx, and a subtype D recombinant present in the population. Four of 13 (31%) infants had NNRTI resistance mutations--V179I (2 infants), Y181C, and V179E. Intriguingly, subtype G sequences did not have NNRTI mutations but rather carried a Q207N mutation, which may undergo negative selection under drug pressure. Our data suggest wide diversity for vertically transmitted HIV-1 viruses in Nigeria and highlight the potential significance of transmitting rare mutations in subtype G.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Anti-HIV Agents / therapeutic use
  • Female
  • HIV Infections / drug therapy*
  • HIV Infections / prevention & control
  • HIV Infections / transmission
  • HIV Infections / virology
  • HIV Reverse Transcriptase / chemistry
  • HIV Reverse Transcriptase / genetics*
  • HIV-1 / enzymology
  • HIV-1 / genetics*
  • Humans
  • Infant
  • Infectious Disease Transmission, Vertical / prevention & control
  • Molecular Sequence Data
  • Mutation*
  • Nevirapine / therapeutic use*
  • Nigeria
  • Phylogeny
  • Pregnancy
  • Pregnancy Complications, Infectious / drug therapy*
  • Pregnancy Complications, Infectious / virology
  • Recombination, Genetic
  • Reverse Transcriptase Inhibitors / therapeutic use*
  • Sequence Alignment

Substances

  • Anti-HIV Agents
  • Reverse Transcriptase Inhibitors
  • Nevirapine
  • reverse transcriptase, Human immunodeficiency virus 1
  • HIV Reverse Transcriptase

Associated data

  • GENBANK/EF394306
  • GENBANK/EF394307
  • GENBANK/EF394308
  • GENBANK/EF394309
  • GENBANK/EF394310
  • GENBANK/EF394311
  • GENBANK/EF394312
  • GENBANK/EF394313
  • GENBANK/EF394314
  • GENBANK/EF394315
  • GENBANK/EF394316
  • GENBANK/EF394317
  • GENBANK/EF394318
  • GENBANK/EF394319