LC MALDI-TOF MS/MS and LC ESI FTMS analyses of HLA-B27 associated peptides isolated from peripheral blood cells

Eva Stodůlková; Petr Novák; Sören-Oliver Deininger; Petr Man; Jana Capková; Daniel Kavan; Eva Ivasková; Miroslav Flieger

doi:10.1016/j.imlet.2007.11.011

LC MALDI-TOF MS/MS and LC ESI FTMS analyses of HLA-B27 associated peptides isolated from peripheral blood cells

Immunol Lett. 2008 Feb 15;116(1):79-85. doi: 10.1016/j.imlet.2007.11.011. Epub 2007 Dec 7.

Authors

Eva Stodůlková¹, Petr Novák, Sören-Oliver Deininger, Petr Man, Jana Capková, Daniel Kavan, Eva Ivasková, Miroslav Flieger

Affiliation

¹ Department of Biogenesis and Biotechnology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídenská 1083, 14220 Prague 4, Czech Republic.

PMID: 18160138
DOI: 10.1016/j.imlet.2007.11.011

Abstract

Peptides eluted from peripheral blood cells of HLA-B*2705 healthy donor were analyzed by LC MALDI MS/MS and LC ESI FTMS techniques. The sequences of 92 peptide ligands identified from one healthy blood donor by LC MALDI-TOF MS/MS were compared with those previously published from in vitro long-term cell cultures available in SYFPEITHI database and splenocytes. It was found that 18 sequences confirmed within 1ppm mass error by LC ESI FTMS were already described and 3 of them matched with those previously reported from HLA-B*2705 splenocytes. Another 38 sequences validated within the same mass error were not found in SYFPEITHI database and are identified here for the first time. Finally, 36 sequences (5 sequences already published in SYFPEITHI database) were evaluated by LC MALDI-TOF MS/MS but no matches in the list of monoisotopic masses obtained from LC ESI FTMS were found.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Alcohol Oxidoreductases
Autoimmunity / genetics
Blood Cells / immunology
Blood Cells / metabolism
Databases, Protein
Endopeptidases / metabolism
GTP-Binding Protein alpha Subunits, Gs / blood
GTP-Binding Protein alpha Subunits, Gs / genetics
GTP-Binding Protein alpha Subunits, Gs / immunology
Genetic Predisposition to Disease
HLA-B27 Antigen / analysis*
HLA-B27 Antigen / immunology
HLA-B27 Antigen / isolation & purification
HSC70 Heat-Shock Proteins / blood
HSC70 Heat-Shock Proteins / genetics
HSC70 Heat-Shock Proteins / immunology
Histones / genetics
Histones / metabolism
Humans
Male
Peptide Mapping*
Peptides / analysis*
Peptides / immunology
Peptides / isolation & purification
Protein Interaction Domains and Motifs
Sequence Alignment
Software
Spectrometry, Mass, Electrospray Ionization*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*
Spondylitis, Ankylosing / genetics
Spondylitis, Ankylosing / metabolism

Substances

HLA-B27 Antigen
HSC70 Heat-Shock Proteins
HSPA8 protein, human
Histones
Peptides
Alcohol Oxidoreductases
D-2-hydroxyacid dehydrogenase
Endopeptidases
GTP-Binding Protein alpha Subunits, Gs