Abstract
The aim of this study was to characterize biomarker responses in three-spined sticklebacks exposed to prochloraz (Pcz). For this purpose, adult sticklebacks were exposed for 2 weeks to prochloraz at 0, 10, 50, 100 and 500 microg/L prior to one week of depuration in clean water. At days 7, 14 and 21, several hepatic biomarkers were measured including 7-ethoxyresorufin-O-deethylase (EROD), glutathione-S-transferase (GST), glutathione peroxidase (GPx), catalase (CAT), total glutathione (GSH) content and thiobarbituric acid reactive substances (TBARS). Pcz induced a transient increase of antioxidant enzymes and a depletion of glutathione content during the first 7 days of exposure. This study showed that EROD activity and antioxidants were disrupted in a transient manner. GST was rapidly induced in a dose-dependent manner and this induction was persistent and observed also after depuration. GST appeared as a valuable biomarker to assess the exposure to Pcz.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biomarkers / analysis
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Biomarkers / metabolism
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Catalase / analysis
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Catalase / metabolism
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Cytochrome P-450 CYP1A1 / analysis
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Cytochrome P-450 CYP1A1 / metabolism
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Dose-Response Relationship, Drug
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Environmental Monitoring / methods
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Fungicides, Industrial / toxicity*
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Glutathione / analysis
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Glutathione / metabolism
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Glutathione Peroxidase / analysis
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Glutathione Peroxidase / metabolism
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Glutathione Transferase / analysis
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Glutathione Transferase / metabolism
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Imidazoles / toxicity*
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Liver / enzymology*
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Liver / metabolism
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Oxidative Stress / drug effects*
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Smegmamorpha / growth & development*
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Thiobarbituric Acid Reactive Substances / analysis
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Thiobarbituric Acid Reactive Substances / metabolism
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Time Factors
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Water Pollutants, Chemical / toxicity
Substances
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Biomarkers
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Fungicides, Industrial
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Imidazoles
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Thiobarbituric Acid Reactive Substances
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Water Pollutants, Chemical
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prochloraz
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Catalase
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Glutathione Peroxidase
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Cytochrome P-450 CYP1A1
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Glutathione Transferase
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Glutathione