Abstract
We screened a series of RNA and DNA aptamers for their ability to serve in the dye displacement assays in which analytes compete with TO dye. We conclude that, while the performance of the TO dye displacement approach is not always predictable, it is still a simple and sensitive assay to detect binding between RNA aptamers and small molecules. In particular, we describe efficient assays for tobramycin and theophylline, with up to 90% displacement of TO observed, and we describe the first aptameric assay for cAMP.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Adenosine Triphosphate / analysis
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Aptamers, Nucleotide / chemistry*
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Benzothiazoles / chemistry*
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Cyclic AMP / analysis
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Fluorescent Dyes / chemistry*
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Guanosine Triphosphate / analysis
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Nucleic Acid Conformation
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Quinolines / chemistry*
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Sensitivity and Specificity
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Theophylline / analysis
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Tobramycin / analysis
Substances
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Aptamers, Nucleotide
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Benzothiazoles
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Fluorescent Dyes
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Quinolines
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thiazole orange
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Guanosine Triphosphate
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Adenosine Triphosphate
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Theophylline
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Cyclic AMP
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Tobramycin