Evaluation of physiological alterations of the placenta through analysis of cell-free messenger ribonucleic acid concentrations of angiogenic factors

Am J Obstet Gynecol. 2008 Jan;198(1):124.e1-7. doi: 10.1016/j.ajog.2007.06.079.

Abstract

Objective: Placental messenger ribonucleic acid (mRNA) has been shown to circulate in maternal plasma. We investigated concentrations of vascular endothelial growth factor (VEGF), VEGF receptor-1 (VEGFR-1), and endoglin in subjects with preeclampsia, compared with normal pregnancies.

Study design: Peripheral blood samples were obtained from preeclampsia (n = 43) and control subjects (n = 41). Plasma ribonucleic acid was subjected to analysis by reverse transcription-polymerase chain reaction assay to examine the mRNA distribution among women with preeclampsia and control subjects during weeks 35-41 of gestation.

Results: Concentrations of VEGF, VEGFR-1, and endoglin mRNA of women with preeclampsia were significantly increased. The mRNA values were observed to correlate directly with the severity of hypertension and proteinuria. VEGFR-1 mRNA was markedly elevated in women with preeclampsia and hemolysis, elevated liver enzymes, and low platelet syndrome.

Conclusion: The mRNA concentrations of VEGF, VEGFR-1, and endoglin were observed to correlate directly with the severity of preeclampsia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Angiogenesis Inducing Agents / blood*
  • Biomarkers / blood
  • Case-Control Studies
  • Disease Progression
  • Female
  • Humans
  • Middle Aged
  • Placenta / physiopathology
  • Pre-Eclampsia / blood
  • Pre-Eclampsia / physiopathology*
  • Pregnancy
  • Probability
  • RNA / blood
  • RNA, Messenger / blood*
  • Reference Values
  • Risk Assessment
  • Sensitivity and Specificity
  • Severity of Illness Index
  • Statistics, Nonparametric
  • Vascular Endothelial Growth Factor A / blood*
  • Vascular Endothelial Growth Factor Receptor-1 / blood*

Substances

  • Angiogenesis Inducing Agents
  • Biomarkers
  • RNA, Messenger
  • Vascular Endothelial Growth Factor A
  • RNA
  • Vascular Endothelial Growth Factor Receptor-1