How long does it take to "rule out" bacteremia in children with central venous catheters?

Pediatrics. 2008 Jan;121(1):135-41. doi: 10.1542/peds.2007-1387.

Abstract

Background: Children with central venous catheters and suspected bloodstream infection are often hospitalized for 48 hours to receive empiric antibiotic therapy pending blood-culture results. Continuous monitoring blood-culture systems allow for more rapid detection of bloodstream infection than previous blood-culture systems, a feature that may facilitate earlier determination of the true presence or absence of bloodstream infection and shorten empiric antibiotic therapy and duration of hospitalization.

Methods: This retrospective cohort study included children with central venous catheters who were diagnosed with laboratory-confirmed bloodstream infection after evaluation in the ambulatory care setting.

Results: Two-hundred episodes of bloodstream infection were included. The median patient age was 5.5 years. Central venous catheters were in place for a median of 80.5 days. Gram-negative bacteria accounted for 51% of infections as part of either a monomicrobial (25%) or polymicrobial (26%) infection. The overall median time to blood-culture positivity was 14 hours. The predicted probability for a culture being positive at 36 hours was 99.2% for infections caused by gram-negative bacteria and 96.6% for any infection after adjusting for age, catheter type, and recent antibiotic use. In a multivariate Cox proportional-hazards regression model, polymicrobial infections with > or = 1 gram-negative bacteria and monomicrobial infections caused by gram-negative bacteria were independently associated with an earlier time to blood-culture positivity after adjusting for age, catheter type, and recent antibiotic use.

Conclusions: The time to blood-culture positivity depends on bacterial category. Bloodstream infections caused by gram-negative bacteria are detected most quickly. Our data suggest that discontinuation of empiric antibiotic coverage may be warranted in clinically stable children with central venous catheters if the blood-culture results remain negative 24 to 36 hours after collection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteremia / diagnosis*
  • Bacteremia / etiology*
  • Bacteriological Techniques / methods
  • Blood / microbiology*
  • Catheterization, Central Venous / adverse effects*
  • Catheterization, Central Venous / instrumentation*
  • Catheters, Indwelling / microbiology
  • Child
  • Child, Preschool
  • Cohort Studies
  • Early Diagnosis
  • Equipment Contamination
  • Female
  • Gram-Negative Bacteria / isolation & purification
  • Gram-Positive Bacteria / isolation & purification
  • Hospitals, Pediatric
  • Humans
  • Infant
  • Infection Control
  • Logistic Models
  • Male
  • Multivariate Analysis
  • Philadelphia
  • Predictive Value of Tests
  • Probability
  • Proportional Hazards Models
  • Retrospective Studies
  • Time Factors