Protein phosphatase 1-dependent bidirectional synaptic plasticity controls ischemic recovery in the adult brain

J Neurosci. 2008 Jan 2;28(1):154-62. doi: 10.1523/JNEUROSCI.4109-07.2008.

Abstract

Protein kinases and phosphatases can alter the impact of excitotoxicity resulting from ischemia by concurrently modulating apoptotic/survival pathways. Here, we show that protein phosphatase 1 (PP1), known to constrain neuronal signaling and synaptic strength (Mansuy et al., 1998; Morishita et al., 2001), critically regulates neuroprotective pathways in the adult brain. When PP1 is inhibited pharmacologically or genetically, recovery from oxygen/glucose deprivation (OGD) in vitro, or ischemia in vivo is impaired. Furthermore, in vitro, inducing LTP shortly before OGD similarly impairs recovery, an effect that correlates with strong PP1 inhibition. Conversely, inducing LTD before OGD elicits full recovery by preserving PP1 activity, an effect that is abolished by PP1 inhibition. The mechanisms of action of PP1 appear to be coupled with several components of apoptotic pathways, in particular ERK1/2 (extracellular signal-regulated kinase 1/2) whose activation is increased by PP1 inhibition both in vitro and in vivo. Together, these results reveal that the mechanisms of recovery in the adult brain critically involve PP1, and highlight a novel physiological function for long-term potentiation and long-term depression in the control of brain damage and repair.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Brain Ischemia / pathology*
  • Brain Ischemia / physiopathology*
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Dose-Response Relationship, Radiation
  • Doxycycline / administration & dosage
  • Electric Stimulation / methods
  • Enzyme Inhibitors / pharmacology
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology
  • Glucose / deficiency
  • Hippocampus / drug effects
  • Hippocampus / physiopathology
  • Hippocampus / radiation effects
  • Hypoxia / complications
  • In Vitro Techniques
  • Long-Term Synaptic Depression / physiology
  • Long-Term Synaptic Depression / radiation effects
  • Mice
  • Mice, Inbred C57BL
  • Neuronal Plasticity / physiology*
  • Protein Phosphatase 1 / physiology*
  • Proteins / genetics
  • Pyrans / pharmacology
  • Recovery of Function / drug effects
  • Recovery of Function / physiology*
  • Recovery of Function / radiation effects
  • Spiro Compounds / pharmacology

Substances

  • Enzyme Inhibitors
  • Proteins
  • Pyrans
  • Spiro Compounds
  • phosphoprotein phosphatase inhibitor 1
  • tautomycin
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2
  • Protein Phosphatase 1
  • Glucose
  • Doxycycline