Differential effects of all-trans retinoic acid on the growth of human keratinocytes and mouth carcinoma epidermoid cultures. Involvement of GRIM-19 and complex I of the respiratory chain

Int J Immunopathol Pharmacol. 2007 Oct-Dec;20(4):719-29. doi: 10.1177/039463200702000407.

Abstract

Squamous cell carcinoma (SSC) is the most frequent malignant tumor of the oral cavity. A study on the effect of all-trans retinoic acid (RA) on cell growth, expression of GRIM-19 and content and activity of complex I of the mitochondrial respiratory chain in normal human keratinocytes (NHEK) and mouth carcinoma cells with low (HN) and high (KB) transformation grade was carried out. In NHEK cells, RA treatment resulted in growth suppression, significant overexpression of GRIM-19 protein, enhanced content of complex I but depressed activity of NADH-UQ oxidoreductase activity of the complex. In HN cells, RA treatment depressed cell growth, inhibited the enzymatic activity of complex I but had no significant effect on the levels of GRIM-19 and complex I. In KB cells RA had no effect on cell growth, GRIM-19 expression, content and activity of complex I.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects
  • Apoptosis Regulatory Proteins / antagonists & inhibitors
  • Apoptosis Regulatory Proteins / metabolism
  • Apoptosis Regulatory Proteins / physiology*
  • Carcinoma, Squamous Cell / pathology*
  • Cell Division / drug effects
  • Cell Line
  • Cell Line, Tumor
  • Cytochromes c / metabolism
  • Electron Transport / physiology*
  • Electron Transport Complex I / antagonists & inhibitors
  • Electron Transport Complex I / metabolism
  • Electron Transport Complex I / physiology*
  • Electrophoresis, Polyacrylamide Gel
  • Glutathione Peroxidase / metabolism
  • Humans
  • Keratinocytes / drug effects*
  • Keratolytic Agents / pharmacology*
  • Molecular Sequence Data
  • Mouth Neoplasms / pathology*
  • NADH, NADPH Oxidoreductases / antagonists & inhibitors
  • NADH, NADPH Oxidoreductases / metabolism
  • NADH, NADPH Oxidoreductases / physiology*
  • Subcellular Fractions / drug effects
  • Subcellular Fractions / metabolism
  • Superoxide Dismutase / metabolism
  • Tretinoin / pharmacology*

Substances

  • Antineoplastic Agents
  • Apoptosis Regulatory Proteins
  • Keratolytic Agents
  • Tretinoin
  • Cytochromes c
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • NADH, NADPH Oxidoreductases
  • NDUFA13 protein, human
  • Electron Transport Complex I