The itinerary of autophagosomes: from peripheral formation to kiss-and-run fusion with lysosomes

Traffic. 2008 Apr;9(4):574-87. doi: 10.1111/j.1600-0854.2008.00701.x. Epub 2008 Jan 7.

Abstract

Macroautophagy, a constitutive process in higher eukaryotic cells, mediates degradation of many long-lived proteins and organelles. The actual events occurring during the process in the dynamic system of a living cell have never been thoroughly investigated. We aimed to develop a live-cell assay in which to follow the complete itinerary of an autophagosome. Our experiments show that autophagosomes are formed randomly in peripheral regions of the cell. They then move bidirectionally along microtubules, accumulating at the microtubule-organizing centre, in a similar way to lysosomes. Their centripetal movement is dependent on the motor protein dynein and is important for their fusion with lysosomes. Initially, autophagosomes dock on to lysosomes, independent of lysosomal acidification. Two kinds of fusion then occur: complete fusions, creating a hybrid organelle, or more often kiss-and-run fusions, i.e. transfer of some content while still maintaining two separate vesicles. Surprisingly, the autophagolysosomal compartment seems to be more long lived than expected. Our study documents many aspects of autophagosome behaviour, adding to our understanding of the mechanism and control of autophagy. Indeed, although the formation of autophagosomes is completely different from any other vesicular structures, their later itinerary appears to be very similar to those of other trafficking pathways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibiotics, Antineoplastic / metabolism
  • Autophagy / physiology*
  • Biological Assay
  • Cells, Cultured
  • Dyneins / genetics
  • Dyneins / metabolism
  • Humans
  • Intracellular Membranes / metabolism
  • Lysosomes / metabolism*
  • Membrane Fusion / physiology*
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism
  • Microtubules / metabolism
  • Molecular Motor Proteins / metabolism
  • Phagosomes / metabolism*
  • Rats
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sirolimus / metabolism

Substances

  • Antibiotics, Antineoplastic
  • LC3 protein, rat
  • Microtubule-Associated Proteins
  • Molecular Motor Proteins
  • Recombinant Fusion Proteins
  • Dyneins
  • Sirolimus