High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents

Proc Natl Acad Sci U S A. 2008 Jan 15;105(2):698-703. doi: 10.1073/pnas.0708338105. Epub 2008 Jan 8.

Abstract

A cell delivery strategy was investigated that was hypothesized to enable magnetic targeting of endothelial cells to the steel surfaces of intraarterial stents because of the following mechanisms: (i) preloading cells with biodegradable polymeric superparamagnetic nanoparticles (MNPs), thereby rendering the cells magnetically responsive; and (ii) the induction of both magnetic field gradients around the wires of a steel stent and magnetic moments within MNPs because of a uniform external magnetic field, thereby targeting MNP-laden cells to the stent wires. In vitro studies demonstrated that MNP-loaded bovine aortic endothelial cells (BAECs) could be magnetically targeted to steel stent wires. In vivo MNP-loaded BAECs transduced with adenoviruses expressing luciferase (Luc) were targeted to stents deployed in rat carotid arteries in the presence of a uniform magnetic field with significantly greater Luc expression, detected by in vivo optical imaging, than nonmagnetic controls.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aorta / cytology
  • Aorta / pathology
  • Biocompatible Materials / chemistry
  • Carotid Arteries / pathology
  • Cattle
  • Cell- and Tissue-Based Therapy / methods
  • Endothelial Cells / metabolism*
  • Genetic Therapy / methods
  • Magnetics
  • Male
  • Metal Nanoparticles / chemistry*
  • Models, Biological
  • Nanotechnology / methods
  • Polymers / chemistry
  • Rats
  • Rats, Sprague-Dawley
  • Steel / chemistry*
  • Stents

Substances

  • Biocompatible Materials
  • Polymers
  • Steel