Abstract
The MET tyrosine kinase receptor activated by its ligand HGF/SF, induces several cellular responses, including survival. Nonetheless, the MET receptor is cleaved in stress conditions by caspases within its intracellular region, generating a 40kDa fragment, p40 MET, with pro-apoptotic properties. Here, we established that this cleavage splits the receptor at the juxtamembrane ESVD site, causing the concomitant generation of p100 MET, corresponding to the entire extracellular region of the MET receptor still spanning the membrane. This fragment is able to bind HGF/SF and to prevent HGF-dependent signaling downstream of full MET, demonstrating its function as a decoy receptor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis / drug effects
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Caspases / metabolism*
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Cell Fractionation
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Cells, Cultured
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Dogs
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Gene Transfer Techniques
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Hepatocyte Growth Factor / antagonists & inhibitors
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Hepatocyte Growth Factor / chemistry
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Hepatocyte Growth Factor / metabolism*
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Humans
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Peptide Fragments / biosynthesis*
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Peptide Fragments / genetics
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Peptide Fragments / pharmacology
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Proto-Oncogene Proteins c-met / chemistry
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Proto-Oncogene Proteins c-met / genetics
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Proto-Oncogene Proteins c-met / metabolism*
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Signal Transduction / drug effects
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TNF-Related Apoptosis-Inducing Ligand / pharmacology
Substances
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Peptide Fragments
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TNF-Related Apoptosis-Inducing Ligand
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TNFSF10 protein, human
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Hepatocyte Growth Factor
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Proto-Oncogene Proteins c-met
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Caspases