Abstract
In invertebrates, the JAK-STAT signaling pathway is involved in the anti-bacterial response and is part of an anti-viral response in Drosophila. In this study, we show that two STAT transcripts are generated by alternative splicing and encode two isoforms of Sf-STAT with different C-terminal ends. These two isoforms were produced and purified using the recombinant baculovirus technology. Both purified isoforms showed similar DNA-binding activity and displayed weak but significant transactivation potential toward a Drosophila promoter that contained a STAT-binding motif. No significant activation of the Sf-STAT protein in Sf9 cells was found by infection with baculovirus AcMNPV.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Cell Line
-
Cell Nucleus / metabolism
-
DNA / metabolism
-
Gene Expression*
-
Humans
-
Molecular Sequence Data
-
Phylogeny
-
Promoter Regions, Genetic / genetics
-
Protein Binding
-
Protein Isoforms / chemistry
-
Protein Isoforms / genetics
-
Protein Isoforms / isolation & purification
-
Protein Isoforms / metabolism
-
STAT Transcription Factors / chemistry
-
STAT Transcription Factors / genetics
-
STAT Transcription Factors / isolation & purification
-
STAT Transcription Factors / metabolism*
-
Sequence Alignment
-
Sequence Homology, Amino Acid
-
Spodoptera
-
Transcriptional Activation / genetics
-
raf Kinases / genetics
-
raf Kinases / metabolism
Substances
-
Protein Isoforms
-
STAT Transcription Factors
-
DNA
-
raf Kinases
Associated data
-
GENBANK/AF329946
-
GENBANK/AF329947
-
GENBANK/AF436838