Interleukin-27 upregulates major histocompatibility complex class II expression in primary human endothelial cells through induction of major histocompatibility complex class II transactivator

Xiao Ming Feng; Xiao Li Chen; Na Liu; Zhong Chen; Yu Ling Zhou; Zhi Bo Han; Lei Zhang; Zhong Chao Han

doi:10.1016/j.humimm.2007.10.004

Interleukin-27 upregulates major histocompatibility complex class II expression in primary human endothelial cells through induction of major histocompatibility complex class II transactivator

Hum Immunol. 2007 Dec;68(12):965-72. doi: 10.1016/j.humimm.2007.10.004. Epub 2007 Nov 20.

Authors

Xiao Ming Feng¹, Xiao Li Chen, Na Liu, Zhong Chen, Yu Ling Zhou, Zhi Bo Han, Lei Zhang, Zhong Chao Han

Affiliation

¹ State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences, Tianjin, China.

PMID: 18191724
DOI: 10.1016/j.humimm.2007.10.004

Abstract

Interleukin-27 (IL-27) is a novel IL-12 family member that plays a critical role in the regulation of T-cell responses. Its immunoregulatory effects on endothelial cells (EC) remain unexplored. Here we show a role for IL-27 in the induction of major histocompatibility complex (MHC) expression in primary human EC. Stimulation of human umbilical vein ECs by IL-27 rapidly induces IFN regulatory factor-1 and dramatically increases the expression of major histocompatibility class II transactivator (CIITA) isoform IV. Expression of this transactivator correlates with increased MHC class II gene expression. IL-27 also enhances expression of MHC class I molecules. Furthermore expression of beta2-microglobulin and transporter associated with antigen processing-1 transcripts increases in response to IL-27. Additional microarray analysis demonstrates that IL-27 significantly upregulates a panel of genes that correlates with immune regulation, including the chemokines CXCL9, CXCL10, and CX3CL1 in human umbilical vein ECs. This first demonstration that both MHC II and I expression are increased in EC after IL-27 stimulation suggests that IL-27 may be important in conferring immune function on vascular endothelium.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ATP Binding Cassette Transporter, Subfamily B, Member 2
ATP-Binding Cassette Transporters / genetics
ATP-Binding Cassette Transporters / metabolism
Cells, Cultured
Chemokines / genetics
Chemokines / immunology
Chemokines / metabolism
Endothelial Cells / immunology*
Endothelial Cells / metabolism
Gene Expression Regulation*
Genes, MHC Class I
Genes, MHC Class II
Histocompatibility Antigens Class II / immunology
Histocompatibility Antigens Class II / metabolism*
Humans
Interferon Regulatory Factor-1 / immunology
Interferon Regulatory Factor-1 / metabolism*
Interleukin-17 / immunology
Interleukin-17 / metabolism*
Nuclear Proteins / immunology
Nuclear Proteins / metabolism*
Oligonucleotide Array Sequence Analysis
RNA, Messenger / genetics
RNA, Messenger / metabolism
Trans-Activators / immunology
Trans-Activators / metabolism*
Umbilical Veins / cytology
Up-Regulation
beta 2-Microglobulin / genetics
beta 2-Microglobulin / immunology
beta 2-Microglobulin / metabolism

Substances

ATP Binding Cassette Transporter, Subfamily B, Member 2
ATP-Binding Cassette Transporters
Chemokines
Histocompatibility Antigens Class II
IRF1 protein, human
Interferon Regulatory Factor-1
Interleukin-17
MHC class II transactivator protein
Nuclear Proteins
RNA, Messenger
TAP1 protein, human
Trans-Activators
beta 2-Microglobulin