Genetic determinants of basal C-reactive protein expression in Filipino systemic lupus erythematosus families

Genes Immun. 2008 Mar;9(2):153-60. doi: 10.1038/sj.gene.6364459. Epub 2008 Jan 24.

Abstract

Basal C-reactive protein (CRP) is a heritable trait associated with long-term cardiovascular disease risk. Existing studies leave ambiguity over the key functional polymorphisms and fail to adjust for trans-acting effects. In a novel cohort of 285 Filipino systemic lupus erythematosus probands and their first-degree relatives, we quantified serum CRP and typed a dense map of CRP single-nucleotide polymorphisms (SNPs), along with SNPs in the interleukin-1 beta, interleukin-6 and apolipoprotein E genes. Ten CRP SNPs demonstrated association with basal CRP in a regression model (P=0.011-0.002). These delineated two haplotypes associated with high and low basal CRP expression (P=0.002). Differences in allele frequency were seen compared with Caucasian populations, enabling us to argue for an independent genetic effect from a phylogenetically distinct haplotype tagged by SNP rs1800947. We demonstrated an association between Apo epsilon 2 and higher basal CRP. Interleukin-6 genotype was associated with basal CRP, highlighting a role for acute-phase cytokines even in basal expression. Identifying these trans-acting variants may improve the use of basal CRP as a predictor cardiovascular risk, and increase our power to detect associations between CRP and disease.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Sequence / genetics
  • Asian People / genetics*
  • C-Reactive Protein / genetics*
  • Cardiovascular Diseases / epidemiology
  • Cardiovascular Diseases / genetics
  • Cohort Studies
  • Female
  • Gene Expression Regulation / genetics*
  • Genetic Linkage / genetics*
  • Haplotypes / genetics
  • Humans
  • Lupus Erythematosus, Systemic / epidemiology
  • Lupus Erythematosus, Systemic / genetics*
  • Male
  • Molecular Sequence Data
  • Pedigree
  • Philippines / epidemiology
  • Risk Factors

Substances

  • C-Reactive Protein