Abstract
In many bacteria, the twin arginine translocation (Tat) system transports folded proteins across the cytoplasmic membrane, and these proteins can play a role in symbiotic or pathogenic infections. A role for the Vibrio fischeri Tat system was identified during symbiotic colonization of its host Euprymna scolopes, demonstrating a function for the Tat system in host colonization by a member of the Vibrionaceae. Using bioinformatics, mutant analyses, and green fluorescent protein fusions, a set of Tat-targeted proteins in V. fischeri was identified.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Aliivibrio fischeri / genetics
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Aliivibrio fischeri / physiology*
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Animals
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Artificial Gene Fusion
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Bacterial Proteins / genetics
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Bacterial Proteins / physiology*
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Decapodiformes / microbiology*
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Gene Deletion
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Genes, Reporter
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Membrane Transport Proteins / genetics
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Membrane Transport Proteins / physiology*
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Virulence Factors / genetics
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Virulence Factors / physiology*
Substances
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Bacterial Proteins
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Membrane Transport Proteins
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Virulence Factors
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Green Fluorescent Proteins