Crystallization and X-ray analysis of Rhodothermus marinus cytochrome c at 1.23 A resolution

Protein Pept Lett. 2007;14(10):1038-40. doi: 10.2174/092986607782541024.

Abstract

Cytochrome c from Rhodothermus marinus has been crystallized using the hanging-drop vapor-diffusion method in 30 % (w/v) polyethylene glycol 8K, 0.2 M ammonium sulfate, 8 % hexanediol and 50 mM sodium citrate pH 2.2. The crystals belong to space group P2(1). X-ray diffraction data were collected to 1.23 A resolution using synchrotron radiation and a wavelength of 0.93 A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallization
  • Crystallography, X-Ray
  • Cytochromes c / chemistry*
  • Hydrogen-Ion Concentration
  • Polyethylene Glycols / chemistry
  • Rhodothermus / enzymology*
  • Synchrotrons

Substances

  • Polyethylene Glycols
  • Cytochromes c