Abstract
The TON_0887 gene was identified as the missing histidinol-phosphate phosphatase (HolPase) in the hyperthermophilic archaeon "Thermococcus onnurineus" NA1. The protein contained conserved motifs of the DDDD superfamily of phosphohydrolase, and the recombinantly expressed protein exhibited strong HolPase activity. In this study, we functionally assessed for the first time the monofunctional DDDD-type HolPase, which is organized in the gene cluster.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Archaeal Proteins / chemistry
-
Archaeal Proteins / genetics
-
Archaeal Proteins / metabolism*
-
DNA, Archaeal / chemistry
-
DNA, Archaeal / genetics
-
Electrophoresis, Polyacrylamide Gel
-
Histidinol-Phosphatase / chemistry
-
Histidinol-Phosphatase / genetics
-
Histidinol-Phosphatase / metabolism*
-
Kinetics
-
Models, Genetic
-
Molecular Sequence Data
-
Multigene Family
-
Sequence Analysis, DNA
-
Sequence Homology, Amino Acid
-
Thermococcus / enzymology*
-
Thermococcus / genetics
Substances
-
Archaeal Proteins
-
DNA, Archaeal
-
Histidinol-Phosphatase