A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis

L J Kenefic; J Beaudry; C Trim; L Huynh; S Zanecki; M Matthews; J Schupp; M Van Ert; P Keim

doi:10.1016/j.mimet.2007.11.014

A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis

J Microbiol Methods. 2008 Jun;73(3):269-72. doi: 10.1016/j.mimet.2007.11.014. Epub 2007 Nov 22.

Authors

L J Kenefic¹, J Beaudry, C Trim, L Huynh, S Zanecki, M Matthews, J Schupp, M Van Ert, P Keim

Affiliation

¹ Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, AZ 86011-5640, United States.

PMID: 18237793
DOI: 10.1016/j.mimet.2007.11.014

Abstract

The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Bacillus anthracis / classification*
Bacillus anthracis / genetics*
Bacterial Typing Techniques / methods*
DNA, Bacterial / genetics*
Electrophoresis, Capillary
Genotype
Polymerase Chain Reaction / methods
Repetitive Sequences, Nucleic Acid*

Substances

DNA, Bacterial