Abstract
Monoclonal anti-collagen type II antibody CIIC1 is an arthritogenic autoantibody, which induces arthritis in mice. We crystallized and solved the structure of CIIC1 Fab molecule. Analysis of structure revealed an interaction between the CDR regions of one Fab to the CH1 domain of another Fab, which resembles an antibody-antigen interaction. ELISA experiments confirmed the cross-reactivity of both the full CIIC1 antibody and a single chain Fv fragment to other anti-collagen antibodies which are of different isotypes and epitope specificity. The rheumatoid factor like reactivity of CIIC1 antibody together with its collagen type II specificity may explain the pathogenicity of this antibody.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Antibodies, Monoclonal / chemistry*
-
Autoantibodies / chemistry*
-
Collagen Type II / chemistry*
-
Complementarity Determining Regions / chemistry
-
Crystallography, X-Ray
-
Enzyme-Linked Immunosorbent Assay
-
Epitopes / chemistry
-
Hydrogen Bonding
-
Immunoglobulin Fab Fragments / chemistry*
-
Immunoglobulin G / chemistry
-
Immunoglobulin Variable Region / chemistry
-
Mice
-
Models, Molecular
-
Molecular Sequence Data
-
Protein Binding
-
Protein Structure, Secondary
-
Sequence Alignment
-
Sequence Analysis, Protein
-
Static Electricity
-
Structure-Activity Relationship
Substances
-
Antibodies, Monoclonal
-
Autoantibodies
-
Collagen Type II
-
Complementarity Determining Regions
-
Epitopes
-
Immunoglobulin Fab Fragments
-
Immunoglobulin G
-
Immunoglobulin Variable Region